Contact an Account ManagerSensitive LC assay for determination of amikacin in human plasma
Summary
Type: HPLC
Application #: 103390
Catalog#: 720140.46 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4.6x150mm
Detection: fluorescence, excitation 340nm, emission 418nm after postcolumn reaction
Conditions: eluent: 0.2M Na2SO4, 0.02M Na pentanesulphonate, 1ml/l acetic acid pH 3.3
flow rate 1.2ml/min
Authors: Wichert, B. et al.
Source: J. Pharm. Biomed. Anal. 9 (1991) 251–254
Details
Legend: Fig. 1. Chromatogram of amikacin (100ng/ml) containing plasma sample, spiked with 200ng/ml tobramycin (int. std.).